Production of recombinant human Phosphoserine Phosphatase
Natascha Weiss, Eppendorf SE
Vincent Dufey, Silvia Tejerina Vargas, Eppendorf Application Technologies S.A.
The production of recombinant proteins by genetically modified organisms is a fundamental biotechnology technique. The goal is to generate a high yield of functional protein which requires largescale cell culture with good cell growth and excellent protein expression. Therefore, a robust shaker is necessary that can shake cultures at high speeds on a liter scale. Centrifugation is used to harvest the cells, to clarify the cell lysate and (if necessary) to concentrate the protein solution.
Harvesting several liters of culture is time-consuming as a result of numerous manual steps required for each individual vessel . For variable culture volumes and further centrifugation steps additional equipment is needed.
In this Application Note, the production of a recombinant protein is presented using a workflow for culturing and harvesting bacteria and purifying proteins. The aim is to demonstrate that a high yield of functional recombinant protein can be realized by using the Innova S44i shaker and a high-speed centrifuge (as a system solution consisting of Harvesting Bundle combined with the High-Speed Pelleting Kit).