Analytik NEWS
Online Laboratory Magazine
08/19/2022

08/04/2022

Production of recombinant human Phosphoserine Phosphatase

Natascha Weiss, Eppendorf SE

Vincent Dufey, Silvia Tejerina Vargas, Eppendorf Application Technologies S.A.

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The production of recombinant proteins by genetically modified organisms is a fundamental biotechnology technique. The goal is to generate a high yield of functional protein which requires largescale cell culture with good cell growth and excellent protein expression. Therefore, a robust shaker is necessary that can shake cultures at high speeds on a liter scale. Centrifugation is used to harvest the cells, to clarify the cell lysate and (if necessary) to concentrate the protein solution.

Harvesting several liters of culture is time-consuming as a result of numerous manual steps required for each individual vessel [1]. For variable culture volumes and further centrifugation steps additional equipment is needed.

In this Application Note, the production of a recombinant protein is presented using a workflow for culturing and harvesting bacteria and purifying proteins. The aim is to demonstrate that a high yield of functional recombinant protein can be realized by using the Innova S44i shaker and a high-speed centrifuge (as a system solution consisting of Harvesting Bundle combined with the High-Speed Pelleting Kit).


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