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Online Laboratory Magazine


Production of recombinant human Phosphoserine Phosphatase

Natascha Weiss, Eppendorf SE

Vincent Dufey, Silvia Tejerina Vargas, Eppendorf Application Technologies S.A.


The production of recombinant proteins by genetically modified organisms is a fundamental biotechnology technique. The goal is to generate a high yield of functional protein which requires largescale cell culture with good cell growth and excellent protein expression. Therefore, a robust shaker is necessary that can shake cultures at high speeds on a liter scale. Centrifugation is used to harvest the cells, to clarify the cell lysate and (if necessary) to concentrate the protein solution.

Harvesting several liters of culture is time-consuming as a result of numerous manual steps required for each individual vessel [1]. For variable culture volumes and further centrifugation steps additional equipment is needed.

In this Application Note, the production of a recombinant protein is presented using a workflow for culturing and harvesting bacteria and purifying proteins. The aim is to demonstrate that a high yield of functional recombinant protein can be realized by using the Innova S44i shaker and a high-speed centrifuge (as a system solution consisting of Harvesting Bundle combined with the High-Speed Pelleting Kit).

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