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Technical papers, application notes and case studies from laboratory, chemistry, life science and quality control

A new generation of optical microscopes for mobile phones
Koen Mortelmans, ChipScope
Optical microscopy is entering a new world. Future microscopes will be chipsised and have super-resolution capabilities. This means they will be available in everyday life, not just in specialist laboratories. And it might become possible to detect SARS-CoV-2 and other viruses using a smartphone....

Bühler Technologies GmbH
Emissions monitoring in shipping
Bühler Technologies GmbH
The emission monitoring is structured like land-based applications and is based on the scope of the exhaust systems to be monitored. Using a heated gas sampling probe, the measurement gas is taken from a suitable point and, in order to avoid the formation of disruptive condensate, is fed to th...

Lodz University of Technology - Institute of Polymer and Dye Technology
Triple Quad LC-MS for detection of luciferin-6'-p-nitrobenzyl ether - a footprint of peroxynitrite
Radoslaw Podsiadly, Aleksandra Grzelakowska, Lodz University of Technology - Institute of Polymer and Dye Technology
Jacek Zielonka, Medical College of Wisconsin

Peroxynitrite (ONOO-), a highly reactive oxidizing and nitrating agent is generated via a spontaneous, diffusion-controlled reaction between nitric oxide (·NO) and superoxide radical anion (O2·-). Peroxynitrite has been implicated as a key pathophysiological intermediate in various diseases, incl...

Bruker Switzerland AG
Automatic Signal Region Detection in 1H NMR Spectra Using Deep Learning
Federico Paruzzo, Simon Bruderer, Christine Bolliger, Bruker Switzerland AG
Signal region detection is a routine task in NMR spectroscopy, however, it is one of the few processing steps that is typically performed manually. This is due to the fact that the method implemented in TopSpin to carry out this task automatically often requires fine-tuning of multiple parameters...

Eppendorf Application Technologies S.A.
Reliable and robust animal-component-free hMSC-BM expansion
Aurélie Tacheny, Wiame Ben El Mostapha, Eppendorf Application Technologies S.A.
Nadine Mellies, Eppendorf SE

In the last decade, human mesenchymal stem cells (hMSCs) have generated increasing scientific interest. Prior to their use as a powerful tool for research applications, hMSCs must be expanded without losing their phenotypic properties. This requires stable and completely defined hMSC culture s...

Wasser 3.0
Investigations on the influence of short-term microplastics exposure on soluble micropollutants in municipal wastewater treatment plants
Michael Sturm, Dennis Schober, Dr. Katrin Schuhen, Wasser 3.0
The water entering sewage treatment plants increasingly poses challenges for operators. More and more often, there is a growing amount of microplastic in rainwater and wastewater for example due to fibers in synthetic clothing such as fleece jackets, tire abrasion or due to the input from industr...

Quality control of silane mixtures with low-field NMR spectroscopy
Simon Kern, Clemens Minnich, S-PACT
In recent years, functional trialkoxy silanes have proven their versatility. After hydrolysis with water or alcohols, modified silanes are applied in numerous applications: from weather protection of buildings to bonding-imparting properties in the glass fiber industry, for sealants and adhesives...

Institute of Analytical Chemistry, Johannes Kepler University
Drug metabolization in plants - Method development for detection of environmentally relevant trace levels
Lisa Emhofer, Institute of Analytical Chemistry, Johannes Kepler University
Due to steady population growth, increasing chronic illnesses and advances in medical science, the consumption of drugs increases continuously. For example, in Germany an increase of 40 - 65% of current demand is expected by 2045. As a negative effect, more and more drug residues are being in...

Eppendorf SE
Optimization of bacterial culture and plasmid purification
Rafal Grzeskowiak, Eppendorf SE, Sandrine Hamels, Blandine Vanbellinghan, Eppendorf Application Technologies S.A.
Plasmid purification from bacterial culture is a commonly performed protocol in molecular biology and life science laboratories. For medium scale preparations, 15 mL conical tubes with screw caps are typically used which often pose handling drawbacks and don't enable achieving optimal productivit...

TU Bergakademie Freiberg
Foam stability and foam structure under high pressure for tertiary oil production - Foam analysis under pressure up to 35 MPa
Martina Szabries, TU Bergakademie Freiberg, Svenja Sergelius, Arne Kordts, Krüss GmbH
Foam properties such as stability, structure, foamability and bubble size distribution depend, among other things, on the type and phase behavior and concentration of the stabilizing surfactant, on the salt content as well as on additives such as polymers and finally on the temperature. When ...