Reliable and robust animal-component-free hMSC-BM expansion

Aurélie Tacheny, Wiame Ben El Mostapha, Eppendorf Application Technologies S.A.

Nadine Mellies, Eppendorf AG

In the last decade, human mesenchymal stem cells (hMSCs) have generated increasing scientific interest. Prior to their use as a powerful tool for research applications, hMSCs must be expanded without losing their phenotypic properties.

This requires stable and completely defined hMSC culture systems consisting of a combination of growth surface and culture medium. Made up of RGD-derived motifs supporting cell attachment, the CCCadvanced FN1 motifs surface from Eppendorf represents a completely synthetic surface for hMSC cultivation in animal-component-free culture conditions.

This surface combines convenience with reliable hMSC cultivation: the ready-to-use consumable significantly reduces labor time and effort for scientists while offering a fully synthetic growth surface with a high level of consistency during long-term hMSC expansion.

hMSCs consist in a heterogeneous population of multipotent cells isolated from various tissues. Their specific properties such as mesodermal differentiation potential, immunomodulation and secretion of anti-in flammatory molecules make them a promising stem cell population in various basic and applied research applications. Present at relatively low abundance in their tissue of origin, hMSCs require a robust in vitro expansion process to obtain sufficient high-quality cell numbers. Traditionally, hMSCs are expanded in vitro in presence of serum on a tissue culture-treated (TCT) surface.

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