Header
Online Laboratory Magazine
07/25/2021

07/16/2020

Reliable and robust animal-component-free hMSC-BM expansion

Aurélie Tacheny, Wiame Ben El Mostapha, Eppendorf Application Technologies S.A.

Nadine Mellies, Eppendorf AG

Share:


In the last decade, human mesenchymal stem cells (hMSCs) have generated increasing scientific interest. Prior to their use as a powerful tool for research applications, hMSCs must be expanded without losing their phenotypic properties.

This requires stable and completely defined hMSC culture systems consisting of a combination of growth surface and culture medium. Made up of RGD-derived motifs supporting cell attachment, the CCCadvanced FN1 motifs surface from Eppendorf represents a completely synthetic surface for hMSC cultivation in animal-component-free culture conditions.

This surface combines convenience with reliable hMSC cultivation: the ready-to-use consumable significantly reduces labor time and effort for scientists while offering a fully synthetic growth surface with a high level of consistency during long-term hMSC expansion.

hMSCs consist in a heterogeneous population of multipotent cells isolated from various tissues. Their specific properties such as mesodermal differentiation potential, immunomodulation and secretion of anti-in flammatory molecules make them a promising stem cell population in various basic and applied research applications. Present at relatively low abundance in their tissue of origin, hMSCs require a robust in vitro expansion process to obtain sufficient high-quality cell numbers. Traditionally, hMSCs are expanded in vitro in presence of serum on a tissue culture-treated (TCT) surface.


» Read article