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Technical papers, application notes and case studies from laboratory, chemistry, life science and quality control

Past, present and future of coliphages - how have these non-pathogenic viruses evolved to become the new water quality indicators?
Dr. Julia Martin, bluephage
More than 80 years ago, the scientific community began to consider coliphages as possible indicators of viral and faecal contamination of water. This article summarises more than forty years of experience presented by Professors Charles Gerba (University of Arizona), Mark Sobsey (University of No...

Rutgers University
A Low Oxygen Atmosphere Supports the Xeno-Free Generation of Human Induced Pluripotent Stem Cells
Rick Cohen, Rutgers University
We previously demonstrated that using low oxygen tension increased the efficiency of reprogramming human somatic cells to pluripotency. In this study, we extend our findings and further increased the development of this culture paradigm. We are able to observe that post-electroporated fibroblasts...

Eppendorf Application Technologies S.A.
Optimizing Plasmid Yields in Shake Culture
Blandine Vanbellinghen, Silvia Tejerina, Eppendorf Application Technologies S.A.
Ines Hartmann, Eppendorf SE

Recombinant plasmid DNA is produced in bacterial cultures, mostly in E. coli. Plasmid yield and quality depend on multiple factors, including the insert, the selection of host strain, the vector design, and the methodology chosen for cultivation and downstream purification. Here, we focus on how ...

Shimadzu Europa GmbH
Plastic determination using fluorescence spectroscopy
Marion Engelkraut-Holtus, Shimadzu Europa GmbH, Albert von Oyen, Erwin Jansen, Carat GmbH
Whether soft drinks, lemonade or sparkling water - PET bottles are used for beverages all over the world. PET stands for "polyethylene terephthalate" and is one of the synthetic polymers commonly known as plastic. Every year, several million tons of polymers are released into the environment. ...

Funke-Dr. N. Gerber Labortechnik GmbH
Simplification of daily laboratory use - Identifying colonies automatically
Nicki Teumer, Funke-Dr. N. Gerber Labortechnik GmbH
Regular colony counts are part of the day-to-day tasks of many laboratories in order to rule out the contamination of products with bacteria, viruses, yeasts, or molds during production. To do this, Petri dishes containing the relevant samples are evaluated in order to determine the number of col...