Thema: Re: Re: Re: Quantifizierung von underivatisierten Aminosäuren mittels LC-MS

Autor(in): Andrew Alpert am 12. April 2006 um 17:18:00

Antwort auf: Re: Re: Quantifizierung von underivatisierten Aminosäuren mittels LC-MS eingetragen von Guido Reineke am 02. Juni 2005 um 22:35:04

In fact, you do not need to use salt gradients routinely in HILIC; just decreasing organic gradients. The exceptions are: 1) When you're running HILIC of extremely hydrophilic compounds such as ATP; 2) When you're using an ion-exchange column in the HILIC mode. That does not pertain to PolyHYDROXYETHYL Aspartamide, though.