Dionex Customer Application Improves Resolution and Separation of Intact Proteins
Dionex announces an improved method for rapid and automated separation of intact proteins from complex samples. Customer Application Note 105: On-Line Two-Dimensional Separation of Intact Proteins describes the use of Dionex ProPac® and Dionex PepSwift™ polystyrene divinylbenzene columns that allow for improved resolution, as well as rapid and automated separation of intact proteins from complex samples.
Off-line two-dimensional separation of proteins is an effective method for simplifying complex samples. However, when using an intermediate fraction collection step, the method has several problems. The on-line two-dimensional method described in Customer Application Note 105 allows more precise control over the gradient and reduces sample loss. In addition, the system allows the possibility of performing entirely automated top-down analysis of complex samples.
Source: Dionex Corp.