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Multiplex analysis with paramagnetic microparticles


Among many challenges, the COVID-19 pandemic also brought a surge of innovation in diagnostics. Within the shortest amount of time, reliable detection methods had to be developed, countless laboratories equipped, and several hundred thousand tests per week carried out.

In the course of this, a team from the Fraunhofer Institute for Laser Technology ILT in Aachen has developed a new readout unit for paramagnetic particles in a microfluidic system.

The special feature here is the type of particles: They are microparticles of different sizes and fluorescence, which are "loaded" with different capture molecules (antigens or antibodies) as required, so that up to 24 analytes can be detected simultaneously.

Many questions have arisen from these challenges in diagnostics: How can tests be scaled more quickly in the future, how can different tests be performed simultaneously? The experts at Fraunhofer ILT and Institut VirionSerion GmbH have addressed the last question in particular in a joint project. They have developed a combination of assay and readout unit that will allow a large number of different tests to be performed simultaneously in the future.

24 channels for clinical multiplex analysis

"A normal flow cytometer is focused on analyzing cells," explains Dr. Georg Meineke from Fraunhofer ILT. "We have developed a system for analyzing microparticles, which we can detect simultaneously over three sizes and different fluorescence levels in 24 different channels." The different species of these particles can be clearly identified via scattered light and fluorescence measurements.

For the actual diagnostics, each particle type can be provided with a specific capture molecule that precisely binds an analyte to be detected. The bound analyte molecules are then detected with a secondary fluorescent marker. In this way, many different diagnostic markers and up to three immunoclasses in case there is proof of antibodies can be detected simultaneously in a single process step.

While the project partner Institut VirionSerion GmbH focused on the particles and the appropriate assay, the researchers at Fraunhofer ILT developed the corresponding microfluidic readout unit. It was trimmed down to a compact design and now includes real-time data processing.

The project partners have built a functional model for an in vitro diagnostic that will later automatically measure particle samples and their associated analytes. This in-vitro diagnostic currently allows the detection of up to 24 individual markers and can also read out up to three different secondary markers in parallel. The electronic platform developed for this purpose controls the measurement system in real time and records the measurement data. It can be integrated into automation solutions such as diagnostic streets.

Source: Fraunhofer Institute for Laser Technology (ILT)