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Online Laboratory Magazine
04/21/2021
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Eppendorf Application Technologies S.A.
Reliable and robust animal-component-free hMSC-BM expansion
Aurélie Tacheny, Wiame Ben El Mostapha, Eppendorf Application Technologies S.A.
Nadine Mellies, Eppendorf AG

In the last decade, human mesenchymal stem cells (hMSCs) have generated increasing scientific interest. Prior to their use as a powerful tool for research applications, hMSCs must be expanded without losing their phenotypic properties. This requires stable and completely defined hMSC culture s...

Eppendorf AG
Optimization of bacterial culture and plasmid purification
Rafal Grzeskowiak, Eppendorf AG, Sandrine Hamels, Blandine Vanbellinghan, Eppendorf Application Technologies S.A.
Plasmid purification from bacterial culture is a commonly performed protocol in molecular biology and life science laboratories. For medium scale preparations, 15 mL conical tubes with screw caps are typically used which often pose handling drawbacks and don't enable achieving optimal productivit...



Eppendorf AG
Is that really DNA in your tube? Comparative analysis of UV-absorbing leachables in micro test tubes
Rafal Grzeskowiak, Diana Hübler, Eppendorf AG
Bioactive contaminants leaching out of laboratory consumables (leachables) may significantly affect experiments and may pose a likely source of error in many assay systems. Recent scientific evidence provides numerous examples of biological assays, which have been shown to be affected by leachabl...

Eppendorf AG
Reduced PCR runtimes and increased yields
Kerstin Isermann, Arora Phang, Eppendorf AG
PCR is one of the most popular methods in laboratories across the world and is routinely used in research, reference and clinical fields for various applications. Over the years, PCR technology has evolved with the needs of scientists; including developments like real-time PCR and digital PCR. ...



Eppendorf Application Technologies S.A.
The Tip of the Iceberg: How Pipette Tips Influence Results - How to Save Money at the Wrong End
Muriel Art, Vincent Dufey, Eppendorf Application Technologies S.A.
Ulrike Gast, Eppendorf AG

With some manufacturers' tips it makes a difference if in serial pipetting (e.g. calibration), a new tip is used for each pipetting or if the series is pipetted using only one tip. This influence on the pipetting result is caused by variations in pipette tip quality. Eppendorf pipette tips were ...

Eppendorf Application Technologies S.A.
The Tip of the Iceberg: How Pipette Tips Influence Results - Not Every Tip Tolerates Every Treatment
Muriel Art, Vincent Dufey, Eppendorf Application Technologies S.A.
Ulrike Gast, Eppendorf AG

The pipetting accuracy of pipette tips by certain manufacturers may suffer after autoclaving. This was not the case for Eppendorf tips. We describe the measures which Eppendorf takes in order to avoid the negative influences of autoclaving. Furthermore, we describe how the interference of biologi...



Eppendorf AG
The Tip of the Iceberg: How Pipette Tips Influence Results - Perfect Geometry Makes a Difference
Ulrike Gast, Eppendorf AG
Laura Koch, Eppendorf Polymere

We performed a study including standard tips from different manufacturers in order to investigate the tip-related influences on the pipetting result. The study showed a dramatic impact of the tip on the pipetting accuracy. Small volume pipetting accuracy is significantly influenced by the quality...

Eppendorf Application Technologies S.A.
The Tip of the Iceberg: How Pipette Tips Influence Results
Muriel Art, Vincent Dufey, Eppendorf Application Technologies S.A.
Ulrike Gast, Eppendorf AG

The fact that a tip fits onto a pipette cone does not say anything about the performance of the pipetting system comprising the components "Pipette and Tip". We performed a study including standard tips from 15 different manufacturers in order to investigate the tip-related influence on the pipet...