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Online Laboratory Magazine
01/26/2021
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Chemisches und Veterinäruntersuchungsamt (CVUA) Stuttgart
Determination of ethyl carbamate in stone fruit spirits, fruit marc spirits and other spirit drinks - A method validation study
Carmen Breitling-Utzmann, Kerstin Zietemann, Simone Goetz, Chemisches und Veterinäruntersuchungsamt (CVUA) Stuttgart
Ethyl carbamate (EC) is a toxic substance which can occur naturally in significant amounts in stone fruit spirits and other spirit drinks. Commission recommendation (EU) 2016/22 states that a target level of 1 mg L-1 EC should be achieved in stone fruit spirits and stone fruit marc spirits. In or...



Chemisches und Veterinäruntersuchungsamt (CVUA) Stuttgart
Determination of Phomopsin A in Lupin-Containing Food, Using UHPLC-MS/MS
Eric Eichhorn, Stefanie Marschik, Chemisches und Veterinäruntersuchungsamt (CVUA) Stuttgart
Phomopsin A, a secondary metabolite of the mold Diaporthe toxica, showed severe toxic effects in animal studies with potential relevance for human health. This lead to the establishment of a legal maximum amount for phomopsin A of 5 µg/kg in lupin seeds and their products by the Australian and Ne...



Chemisches und Veterinäruntersuchungsamt (CVUA) Stuttgart
Analysis of Phosphine in Dried Foodstuffs via Headspace-GC-MSD
Roland Perz, Anne Benkenstein, Ellen Scherbaum, Chemisches und Veterinäruntersuchungsamt (CVUA) Stuttgart
Phosphine is one of the most widely used, cost-effective and rapidly acting fumigants. In EU legislation , maximum residue limits for the sum of phosphine and phosphides in foodstuff are set to within a range of 0.01 and 0.1 mg kg-1, depending on the commodity. A highly sensitive headspace-GC-MSD...

Chemisches und Veterinäruntersuchungsamt (CVUA) Stuttgart
A novel tube-in-tube filter system for refinement of DNA preparation for detection of Mycobacterium using Real-Time PCR
Reinhard Sting, Lisa Schneider-Bühl, Chemisches und Veterinäruntersuchungsamt (CVUA) Stuttgart
Eduard Woizenko, HAMILTON Bonaduz AG

A novel tube-in-tube filter system, the AutoLys M 1.0 tube with a 1 µm pore size non-DNA binding and RNase and DNase free filter designed and produced by HAMILTON Bonaduz AG could be successfully integrated in the procedure of DNA extraction from fecal samples for testing on Mycobacterium avium s...

Chemisches und Veterinäruntersuchungsamt (CVUA) Stuttgart
Identification of Salmonella Enterica Serovar Enteritidis Vaccine Strains by Fourier Transform Infrared Spectroscopy
Dr. Norman Mauder, Dr. Jörg Rau, Chemisches und Veterinäruntersuchungsamt (CVUA) Stuttgart
In cases of salmonellosis in humans, the most frequently isolated serovars of Salmonella enterica are Enteritidis (serogroup D1) and Typhimurium (serogroup B). In order to control the main sources of this zoonotic pathogen, poultry populations are treated with attenuated live vaccines. The distin...



Chemisches und Veterinäruntersuchungsamt (CVUA) Stuttgart
Analysis of Perchlorate in Food Samples of Plant Origin Applying the QuPPe-Method and LC-MS/MS
Ellen Scherbaum, Julia Hepperle, Anne Wolheim, Chemisches und Veterinäruntersuchungsamt (CVUA) Stuttgart
This study reports about how to integrate the environmental contaminant perchlorate into the QuPPe multiresidue method for the analysis of polar pesticides. Perchlorate, along with a number of polar pesticides, was extracted with acidified methanol. The extracts were filtered and subjected to liq...

Chemisches und Veterinäruntersuchungsamt (CVUA) Stuttgart
Determination of quaternary ammonium compound residues in fruits and vegetables by QuEChERS following LC-MS/MS analysis
Ellen Scherbaum, Julia Hepperle, Dr. Eberhard Schüle, Chemisches und Veterinäruntersuchungsamt (CVUA) Stuttgart
A simple and fast method to determine residues of quaternary ammonium compounds (QAC) in fruit and vegetable samples is presented. The analytes were extracted applying the frequently used QuEChERS method. Analysis was performed by liquid chromatography-electrospray tandem mass spectrometry (LC-ES...