Hyphenated HPTLC

The participants

• Understand the meaning of effect-directed analysis as well as advantages and disadvantages of the different techniques
  
• Survey the variety of planar assays (on surface, in situ, in the adsorbent bed)
  
• Know the streamlined workflow on one plate, i.e. parallel separation of compounds in complex samples, discovery of active compounds and their characterization by chromatographic, spectroscopic and spectrometric information (3-20 min/sample for up to 20 samples in parallel)
  
• Recognize the highly efficient combination of planar chromatography with biological and biochemical or other effect-directed assays
  
• Realize the power of hyphenated HPTLC and the unique benefit of effect-directed profiles

Content

• Antimicrobials via Gram-negative Aliivibrio fischeri and Gram-positive Bacillus subtilis bioassays
  
• Enzyme inhibitors via α- and ß-glucosidase, α-amylase, acetyl- and butyrylcholinesterase, tyrosinase and ß-glucuronidase assays
  
• Genotoxic compounds via SOS-Umu-C bioassay, cytotoxic bioassay, neurotoxic bioassay
  
• Endocrine-acting compounds via planar yeast estrogen/androgen screen (pYES/pYAS)
  
• Multiplex planar bioassays: agonistic and antagonistic effect detection (pYAES/pYAAS) with verification (pYAVES/pYAVAS) and detection of synergists → 4-fold multiplex
  
• NanoGIT+active: on-surface simulated static digestive system including separation and effect detection
  
• On-surface metabolization via S9 liver enzyme system
  
• On-surface adhesive/adherent cell assays
• Coupling to HESI-HRMS and DART-MS

Target Group

• Scientists in the field of consumer protection, analytical/environmental/medical chemistry, forensic, doping etc.
  
• Food chemists and analysts in official labs (to learn the powerful priorization strategy)
  
• Producing industries (to learn more about hazards in products)
  
• University staff (to teach on sophisticated HPTLC-EDA)
  
• Researcher in all fields (to better understand complex samples)

» Details

• Science Forum Chemistry 2025 (09/29/2025 - 09/30/2025 conference (Karlsruhe [D]))