Eppendorf

Microinjection in Zebrafish and Medaka: From Transgenesis to CRISPR


Target group:

Research technicians and scientists interested in the generation of genetic modified fish models. Basic experience with molecular biology techniques is required.

Target:

The aim of the course is to provide participants with hands-on tutorials and the theory behind microinjection into medaka and zebrafish. In addition, participants will learn to design CRISPR constructs.

Contents:

  • Microinjection of lineage tracers into one-cell stage embryos
  • Co-injection of DNA and mRNA into one-cell stage embryos
  • Microinjection of CRISPR/Cas constructs
  • Targeted inactivation of pigment loci or genes of interest provided by applicants (medaka and zebrafish)
  • Homology directed repair in fish
  • Screening by imaging
  • High throughput, high content screening for successful events
  • Molecular validation of Crispr/Cas9 mediated indel formation



Organizer:
Eppendorf
Event Type:
seminar
Date:
07/02/2019 - 07/04/2019
City:
Heidelberg
Country:
Germany

Contact:
Eppendorf AG
Kathrin Nölter
Barkhausenweg 1
D-22339 Hamburg

Tel.: +49 40 538 01 361
Fax: +49 40 538 01 840
Mail: Send request Contact
Web: www.eppendorf.com